Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 12th International Conference on Tissue Engineering & Regenerative Medicine Madrid, Spain.

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Scientific Program Day 1

Day 1 :

  • Stem Cell Engineering (SCE) | Tissue Engineering and 3D Printing | Biochips & Tissue Chips | Cancer Stem Cells | Chemotherapy | Tissue Regeneration | Advances in Stem Cell

Session Introduction

Li Han Lin

National Cheng Kung University, Taiwan

Title: In vitro study in functional coatings of biodegradable magnesium-zinc-zirconium alloy for cardiovascular stents
Biography:

Li Han Lin has her well passion in improving the medical implants and wellbeing. She has built this model after years of experience in research, study and crossfield learning in biomedical engineering department and laboratory practice. The foundation is based on new generation for cardiovascular stents. This approach is responsive to create a biomaterial application from lab work to clinical trial.

Abstract:

Vascular stents are important and successful medical device in treating coronary artery disease. Currently, titanium alloy with or without anti-thrombosis drug are the common product. [1] However, the inert metal inside blood vessel could elicit long term inflammation and higher potential of thrombosis. In addition, the drug usage is toxic to the cells in order to inhibit the inflammation caused by the tissue factors released by macrophages.


Degradable polymer PLA was used as the material for the stent fabrication, but the acid degradation product could cause local inflammation and increase the rate of thrombosis. Therefore, using an absorbable magnesium-based stent becomes an ideal solution to long-term endothelial dysfunction. Degradable magnesium alloy has better biocompatibility, mechanical and degradation properties than current PLA base bio-absorbable polymer to serve the stent material. [2] However, Mg alloy without modification cannot provide proper corrosion resistance. Moreover, it was demonstrated that Gallic acid promoted the growth of normal cells [3]. In this study, alkali-heat treatment with two main groups: (1) polymer PLGA (PLGA group)[4] and (2) Gallic acids loaded PLGA (GA group) coating was used to improve corrosion resistance and cell-adhesion.[5] The ZK60 Mg alloy plate samples treated with PLGA and/ or GA dip-coating then were tested by electrochemical test, cell cytotoxicity, and cell adhesion. The results showed GA group has good significant difference in cell vitality, cell adhesion and corrosion resistance compared to control PLGA group (4wt %). In biocompatibility tests, cell has significant vitality in GA group with 1wt% but toxicity with 10wt%, while PLGA group has more cell adhesion on the surface. In electrochemical test, GA group has lowest corrosion density: (1) 6.7474E-08 for 1wt% and (2) 4.989E-08 for 10wt%. It is important to maintain cell vitality and corrosion resistance simultaneously. Therefore, PLGA-4wt%-GA-1wt% dip-coating is an ideal modification applied in vascular stents.

Ying-Jhen Huang

National Cheng Kung University, Taiwan

Title: Novel wound dressings containing magnesium Ions
Biography:

Ying-Jhen Huang is a master student major in biomedical engineering at the National Chung Kung University. She had worked in a medical device company for three years as a researcher. Because the work experience makes her understand the wound healing and the wound care process. In addition, there is a certain understanding of the development of the medical device and regulatory certification. Because she is very interested in tissue engineering, so she decided to invest in research on wound dressing development helping tissue repair.

Abstract:

Care for chronic wounds caused by diabetes and pressure sores is still an important clinical problem. A new stratagem to develop innovative wound dressings is needed. During wound healing, magnesium, zinc, copper, and iron are found to be indispensable elements in wound healing, and magnesium ions are the only one involved in all three stages of wound healing [1]. So far, no research has applied magnesium ions in active wound dressings. This study investigates the potential benefit of a novel Mg ions coating nonwoven PP dressing in wound healing in vitro and in vivo rat model.

The morphology of PP dressing after plasma Mg ions coating at 100 nm in thickness did not found any change by SEM, but the contact angle decreased significantly indicated the hydrophilicity alternation. The immersion test by ICP-MS showed the Mg ions almost completely released in 2 days. In vitro test, the Mg ions solution released from the dressing did not show any toxicity effect compared to positive control. In vivo test, twenty adult male Wistar rats was divided into acute (rat model) and the chronic wound (diabetic rat model) groups. The four 1 cm in diameter full-thickness wound were cut on the back (2 on each side) and covered with Mg ions coating dressing or non-coating dressings. The wound area in Mg ions dressing group shows much faster close pace than the control group (without Mg ions coated wound dressing). From temporal histology results also shows epithelialization ability of the Mg ions dressing group has better performance than the control group. Also, the arrangement of cells and extracellular matrix in histology images also show closer to those of intact skin. In conclusion, Mg ions coated PP nonwoven dressing can significantly improve the process of wound healing on acute and chronic wound. It expected to be a new generation of advanced wound dressings.

Ming Jian

Secondary school of Nankai, China

Title: Bio-osteochondral scaffold stress distribution analysis through finite element method for in vivo test
Biography:

Ming Jian had been in the training team of biology Olympics competition in the high school and have studied the college level biological courses like molecule, cryobiology, biochemistry, botany and plant physiology. Now she is researching on the tissues regeneration instructed by a doctor in UK. She has experienced in modeling using computer technology to help herself design the experiment.

Abstract:

Statement of the Problem: Osteoarthritis (OA) is a disease that occurs commonly among the elder people with adverse impact on their life. However, current treatments depending on replacement of joints and operation will cause the osteochondral defect. Osteochondral scaffold, as regenerative medicine could prevent patients from further deterioration of OA. The various pore sizes influence the growth of the cells, their differentiation and relocation.

Nevertheless, balancing them with the ability of mechanical support also need to be considered. Finite element analysis is used to discover the different functions applied to the osteochondral scaffolds with various pore sizes. Methodology & Theoretical Orientation: Scaffolds are 3-D printed by EOS 290 made of titanium alloy-Ti6Al4V. They are cross-link designed, accumulated by the 0.5 minimum-beam. And the interval of columns is 1 minimum. The scaffolds were put into bull’s knee condyle and all sheep were anesthetic by intravenous injection after 3 months. Bone ingrowth were analyzed through CT and micro CT scanning. Scaffolds mechanical properties were analyzed through the finite element analysis under different situation. It resulted in the changes of stress distribution and deformation applied to the scaffolds.

Findings: According to in vivo tests, we found that tissues prefer to regenerate on the scaffold surface. Stress concentration point showed less tissue than the other parts of the scaffolds.

Conclusion & Significance: As for tissue regeneration, tissue could not regenerate well on the scaffold stress concentration area. And 3D printed titanium alloy scaffold showed good bio-performance.

Ayda Yari Ilkhchi

Azarbaijan Shahid Madani University, Iran

Title: Effects of modified Graphene oxide on nervous tissue regeneration
Biography:

Ayda Yari Ilkhchi is a Ph.D. candidate of Organic Chemistry at Azarbaijan Shahid Madani University, Iran since 2016; working on "Synthesis and Characterization of some biomaterials for treatment and regeneration in Spinal Cord Injuries". Her background is in animal handling, cell culture; and analytical techniques such as UV-Vis spectrophotometry, FTIR, SEM, EDS, TGA, XRD, DSC and ELISA. Her main research interests are in the materials, biomaterial, neuroscience, nanocarrier, polymer science, molecular imprinted polymers, nanocomposites, tissue engineering, novel drug delivery system, regenerative medicine, biosensor and biomedicine.

Abstract:

Nervous system lesions are generally entangling and crucial problems worldwide. Renovation and recovery of nervous system damages, because of their function and anatomy, have been challenging compared with other tissues. The spinal cord injury (SCI) is a portion of central nervous system (CNS); almost two million people suffer from it throughout the world. Recently treatment and regeneration of damaged tissue, sensory recovery and motor function are investigated by different researchers globally. Graphene oxide (GO)-based nanomaterials as considerable materials, with wonderful and precious properties, propose numerous chances for designing pioneer scaffolds in neural tissue engineering to cure the central and peripheral nervous system injuries by neuroregenerative therapies. In this study, GO was synthesized by Modified Hummer method. In order to improve functionality and biocompatibility of GO, was modified with polymers. Follow up chemical analysis; we are investigating in vitro (cell culture) and in vivo (mice model).

The results of analysis show a successful structural and morphological preparation. It is anticipated that Graphene nanomaterials indicate a strong performance in proliferation and differentiation of neural cells, recovery and regeneration of damaged neurites, due to the significant flexibility, extraordinary electrical, mechanical, morphological, and chemical properties.

A S Sotnichenko

University of the Ministry of Healthcare of the Russian Federation, Russia

Title: Morphological evaluation of tissue reaction to subcutaneous implantation of decellularized rat heart and lung matrices
Biography:

A S Sotnichenko is the head of the laboratory for fundamental research in the field of regenerative medicine. He is engaged in the development of methods for obtaining and evaluating decellularized matrices of the heart, lung, diaphragm, trachea, skin and esophagus.

Abstract:

At present time the number of works devoted to the decellularization of various organs has significantly increased. There are many techniques and methods for the preparation of acellular extracellular matrix (ECM), whether based on the physical, chemical and enzymatic effects on the interest. The resulting structure does not contain the saved cells and their decay products, and the complex geometry of the organ, including a relatively intact vasculature, is largely preserved.
 
Methodology: The work was performed on 10 male Wistar rats weighing 210 ± 40 g. Decellularization of the lung and rat heart was performed by modified protocols. The samples were implanted subcutaneously in the interscapular region. Rats were derived from experiment on days 7 and 14. We made a qualitative assessment of the composition of the cellular infiltrate around the implant using immunohistochemistry.
 
Results: Based on the morphological analysis, a histological evaluation of rat tissue response to subcutaneous implantation of the decellularized heart matrices was performed. The qualitative cellular composition of the inflammatory infiltrate was studied with an assessment of the dynamic changes in the macrophages, T- and B-lymphocytes amount on days 7 and 14 after the beginning of the experiment. The results obtained revealed a different tissue response of the recipient organism to ECM implantation. The least expressed was the response to the lung ECM, which successfully integrated into the tissues and did not undergo significant changes. It is shown that he tissue response to implantation depends not only on the quality of decellularization and the efficiency of antigen molecules removal, but also on the initial histological architectonics and quality of preimplantation preparation of the sample.

Jian Xu

Sun Yat-sen University, China

Title: Reduced cell division control protein 42 activity compromises hematopoiesis-supportive function of Fanconi anemia mesenchymal stromal cells
Biography:

Jian Xu is a PhD student at School of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University. Jian’s research focus on molecular analysis of abnormal hematopoiesis, primarily on the molecular pathogenesis of bone marrow failure and leukemia. He has finished his research program at Dr. Wei Du lab, department of Pharmaceutical Sciences, West Virginia University as a visiting student in the last one and half year. His recently work entitled “Mesenchymal COX2-PG secretome engages NR4A-WNT signaling axis in haematopoietic progenitors to suppress anti-leukemia immunity” has been accepted by British Journal of Hematology.

Abstract:

Hematopoietic stem cells preserve their ability to self-renew and differentiate to different lineages in the bone marrow (BM) niche, which is composed in large part by BM stromal cells. Studies have shown that altered signaling in the BM niche results in leukemia initiation or progression. Fanconi anemia (FA) is an inherited BM failure syndrome associated with extremely high risk of leukemic transformation. By using two FA mouse models, here we have investigated the hematopoiesis-supportive function of FA BM mesenchymal stroma cells (MSCs). We found that MSCs deficient for Fanca or Fancc gene are defective in proliferation and prone to undergo senescence in vitro. Mechanistically, we show that the activity of cell division control protein 42 (Cdc42), a Rho GTPase known to be a critical regulator for cytoskeleton organization, is significantly reduced in FA MSCs. Furthermore, we demonstrate that this reduction in Cdc42 activity plays a causal role in defective hematopoiesis-supportive function of the FA MSCs. The progenies of wild-type hematopoietic stem and progenitor cells cocultured on FA MSCs exhibit compromised self renewal capacity both in vitro and in vivo. Genetic correction of FA deficiency restores Cdc42 activity and improves the hematopoiesis-supportive capacity of FA MSC. Finally, ectopic expression of a constitutively active Cdc42 mutant, Cdc42F28L, or pretreatment with Wnt5a, increases the active Cdc42 level and rescues the hematopoietic supportive defects of FA MSCs. Taken together, our results identify a novel link between Cdc42 activity and the hematopoiesis-supportive function of MSCs and suggest that a niche-specific increase of Cdc42 activity may be beneficial for FA therapy.

Stepanova O V

National Medical Research Center for Psychiatry and Narcology, Russia

Title: Efficiency of olfactory mucosa cell combination in the treatment of the spinal cord injuries
Biography:

Stepanova O V works in the field of the regenerative medicine. She studies the resident stem / progenitor neural cells. Now she is actively working in the field of investigation of olfactory mucosa neural stem / progenitor and ensheathing cells. Development of methods for obtaining the cell cultures from the olfactory mucosa and the study of the therapeutic efficacy of these cells in experimental posttraumatic spinal cord cysts will create the preconditions for their successful application in the treatment of patients with posttraumatic cysts of spinal cord.

Abstract:

Statement of the Problem: Pathological processes developing after spinal cord injuries are associated with a functional mature neurons death and severe defects of motor, sensory, respiratory and digestive functions. The most important task of modern biomedicine is to find new treatment strategies for such patients. Promising direction in this area is the cell therapy. The olfactory ensheathing cells and neural stem/progenitor cells (NSPC) from olfactory mucosa are considered optimal. However, the use of a combination of these cells in the treatment of spinal cord injuries has not been studied. Thus, the aim of this work was to study the efficiency of olfactory mucosa cell combination in the treatment of the spinal cord injuries.
 
Methodology & Theoretical Orientation: We have obtained purify cultures of human olfactory ensheathing cells and NSPC. Cyst formation was confirmed by magnetic resonance imaging (MRI) 4 weeks after spinal cord injury. The cell combination was transplanted into the cyst area in 10 μl of DMEM/F12 medium (n=7). The control group was injected with the same amount of medium without cells (n=7). Assessment of motor function recovery was performed within 3 weeks after transplantation using the BBB test. Findings: The optimal cell combination consisted of 0.75 million of olfactory ensheathing cells and 0.2 million of NSPC. We have demonstrated the efficiency of this cell combination to improve mobility of the rat hind limbs.
 
Conclusion & Significance: In this and previous studies, we have shown that olfactory mucosa cell transplantation improves mobility of the hind limbs of rats. Further research in this area will help to develop a combined use of cell therapy with surgical and therapeutic strategies for the treatment of patients with spinal cord injuries. The study was supported by the Russian Science Foundation (grant No. 17-15-01133).

Tarek K Motawi

Cairo University, Egypt

Title: Chemomodulatory effect of betanin against paracetamol and diclofenac induced neurotoxicity and endocrine disruption in rats
Biography:

Tarek K Motawi, Professor of Biochemistry, Faculty of Pharmacy, Cairo University. Egyptian, date of birth 6/3/1955. Ph.D. in Pharmaceutical Sciences, 1984; M.Sc. in Pharmaceutical Sciences, 1979; B.Sc. in Pharmaceutical Sciences, Faculty of Pharmacy, Cairo University, 1976. Professional experience: Instructor; 1976, Lecturer Assistant; 1980, Lecturer, 1984; Assistant Professor, 1989; Professor, 1994; Head of the Department of Biochemistry, Faculty of Pharmacy, Cairo:- 2008-2014.

Abstract:

Paracetamol and diclofenac are two of the most popular analgesics and anti-inflammatory medications. Despite of their several therapeutic benefits, their over consumption led to subsequent cellular damage. Their cytotoxicity is attributed to reactive radical generation. Betanin has antioxidant and anti-inflammatory properties. The protective effects of betanin against paracetamol or diclofenac induced neurotoxicity or endocrine disruption has not been investigated before. Therefore, this study aims to explore the protective potential of betanin against paracetamol or diclofenac neurotoxicity and endocrine disruption in a rat model. In brain, paracetamol (400 mg/ kg) and diclofenac (10mg/kg) enhanced DNA fragmentation and lipid peroxidation level. A depletion of GSH content concomitant with a reduction in the activities of antioxidant enzymes (HOX-1, POX-1, CAT and SOD) were detected. Serotonin, nor-adrenaline and dopamine levels were markedly reduced after paracetamol and diclofenac challenge. In serum, a significant reduction of testosterone, TRH, TSH, T3 and T4 were associated with the enhanced oxidative damage. Co-treatment of rats with betanin (25mg/kg) by gavage for 28 consecutive days ameliorated most of the biochemical and histopathological changes induced by paracetamol or diclofenac. In conclusion, betanin exerted a potential chemomodulatory effect against paracetamol or diclofenac overconsumption induced neurotoxicity and endocrine disruption.

A S Sotnichenko

University of the Ministry of Healthcare of the Russian Federation, Russia

Title: Development of method for obtaining the dermal extracellular matrix
Biography:

A S Sotnichenko is the head of the laboratory for fundamental research in the field of regenerative medicine. He is engaged in the development of methods for obtaining and evaluating decellularized matrices of the heart, diaphragm, trachea, skin and esophagus.

Abstract:

Statement of the Problem: Despite the achievements of modern surgery in the treatment of cutaneous injuries, the search for new methods for faster and more effective wound healing remains topical. Tissue engineering is undoubtedly of interest for the development of such technologies.
 
Aim: of the study was to determine the optimal protocol for obtaining a decellularized dermal matrix for the subsequent development of tissue-engineered skin.
 
Materials and Methods: The experimental animal was 1 pig of the Landrace breed. After skin pretreatment with a dermatome, the samples were taken of a thickness of 0.3 cm. Two decellularization protocols were examined: protocol No.1 based on the using of Triton X-100 and deoxycholate, protocol No.2 only based on deoxycholate. Total processing cycles for 2 protocols were 5. The cellular matrices after treatment were examined with: histological analysis, quantitative determination of DNA content in wet tissue. Further, static matrix recellularization of porcine dermis fibroblasts was carried out. After that, the matrices were assessed for cytotoxicity using an XTT test and a test for the differential staining of living and dead cells.
 
Results: The comparative analysis of two protocols for decellularization of porcine dermis showed that both protocols effectively remove cells and nuclear material, while maintaining the architectonic of the intercellular substance intact, since fibrous structures are not destroyed. But when analyzed the functional properties of matrices on the basis of cell viability analysis according to the XTT test and cell adhesion to the matrix, the matrix processed according to protocol No.1 demonstrates the advantages.
 
Conclusion: In this study, a decellularization protocol based on Triton X-100 and deoxycholate was noted. The results are the first stage for the further development of tissue-engineered skin.